The detection of non-RoTat 1.2 Trypanosoma evansi

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Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections

BACKGROUND: Based on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. A total of 39 different trypanosome stocks were tested using the RoTat 1.2 based Polymerase Chain Reaction (PCR). RESULTS: This PCR yielded a 205 bp product in ...

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ژورنال

عنوان ژورنال: Experimental Parasitology

سال: 2005

ISSN: 0014-4894

DOI: 10.1016/j.exppara.2005.01.001